Improved detection of human influenza A and B viruses in respiratory tract specimens by hemi-nested PCR
نویسندگان
چکیده
منابع مشابه
Detection of Human Influenza Viruses in Nasopharyngeal Samples by RT-PCR vs Tissue Culture
Background and Aims: Influenza virus is a major pathogen involved in respiratory illnesses during winter seasons. A variety of diagnostic methods have been developed to identify influenza viruses in clinical specimen. Methods: Nasal and pharyngeal samples taken from patients were inoculated into Madin-Darby canine kidney (MOCK) cells and embryonated chicken eggs (ECEs). The culture media was a...
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Human papillomaviruses (HPVs) infect mucosal and cutaneous epithelia and cause malignant and benign neoplastic lesions. Human papillomaviruses cause 250000 deaths per year from cervical cancer most often in developing countries. This major public health problem make them important targets in the search for Human papillomaviruses detection methods that may play a role in cervical cancer screen...
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Respiratory tract infections can be caused by a heterogeneous group of viruses and bacteria that produce similar clinical presentations. Specific diagnosis therefore relies on laboratory investigation. This study developed and evaluated five groups of multiplex nested PCR assays that could simultaneously detect 21 different respiratory pathogens: influenza A virus (H1N1, H3N2, and H5N1); influe...
متن کاملcomparison of multiplex nested rt-pcr with virus isolation for detection of influenza viruses a and b
background: influenza a viruses bearing all known ha and na subtypes have been isolated from avian hosts: but only the a/h1n1,a/h2n2 and a/h3n2 influenza subtypes have been associated with widespread epidemics in humans until now .while influenza b detected only in humans. subtype h5 infection in humans occurred for the first time during 1997-1998 in hong kong, then china in 2003, vietnam and...
متن کاملdetection of human influenza viruses in nasopharyngeal samples by rt-pcr vs tissue culture
background and aims: influenza virus is a major pathogen involved in respiratory illnesses during winter seasons. a variety of diagnostic methods have been developed to identify influenza viruses in clinical specimen. methods: nasal and pharyngeal samples taken from patients were inoculated into madin-darby canine kidney (mock) cells and embryonated chicken eggs (eces). the culture media was as...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2007
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2006.12.010